Comparison of various methods of Extended Spectrum Beta Lactamase detection among Gram negative isolates of family Enterobacteriaceae.

Shuchi Mehra; Rama Sikka; Uma Chaudhary

Volume : VIII, Issue : III, March - 2018

Comparison of various methods of Extended Spectrum Beta Lactamase detection among Gram negative isolates of family Enterobacteriaceae.

Shuchi Mehra, Rama Sikka, Uma Chaudhary

Abstract :

Introduction: ESBL producing members of family Enterobacteriaceae have been responsible for numerous outeaks of infection throughout the world. ESBL detection thus propagates the use of higher end antibiotics.

Material and methods: A total of 100 non repetitive MDR strains isolated from various clinical samples were included in our study. All isolates were identified by standard microbiological techniques and antimicrobial susceptibility pattern was determined. Detection of ESBL production was carried out by three methods: phenotypic confirmatory test (CLSI method), method using sulbactam as inhibitor and chromogenic medium.

Results: ESBL production was seen in 70 (70%) isolates by CLSI method, whereas 68 (68%) isolates showed ESBL production when sulbactam was used as the inhibitory agent. By using chromogenic medium, 77 (77%) out of 100 isolates showed ESBL production.

Conclusion: Chromogenic media are rapid and better screening method for ESBL detection.