Volume : III, Issue : VII, July - 2014

Comparison Study between Antibiogram and Quorum–Sensing (Qs ) Gene Clusters Analysisl for Staphylococcus Aureus

Shaymaa Khudhr Al Alak

Abstract :

Thirty–eights S. aureus isolates gathered from differ¬ent hospitals and isolated from various specimens. All isolates under study design and distributed to three groups depending on the type of multidrug resistance as well as dendrogram based on antimicrobial drug sensitivity pattern of 38 non– repetitive staphylococcal isolates and the accessions are clearly divided into two major clusters, A and B. As to the susceptibility to antibiotics, there was no signi?cant variability between test repetitions. The agr speci?city group was determined by a PCR method analysis of agr gene polymorphism by speci?c primers allowed assigning our strains in 1 of 4 major speci?c agr groups, most of our isolates belonged to agr group I (32%), followed by agr group II (47%), agr group III (16%), and agr group IV (5%). To considering the possible relation between the agr group and the antibiotic resistance in S. aureus isolates, the study showed that isolates with a resistance group A ( table 3)table.4) , the dominant genes from (4 isolates agrII) and One isolate each group agrI and agrIII, But not contain agrIV genes. group B the dominant genes from (5 isolates agrII) and ( 5 isolates agrI) gene and (2 isolates agrIII, 2 isolates agrIV) .the high resistance group C the dominant genes from (9 isolates agrII) and (6 isolates agrI ) ,(3 isolates agrIII) But not contain agrIV genes.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Shaymaa Khudhr Al–Alak Comparison Study between Antibiogram and Quorum-Sensing (Qs ) Gene Clusters Analysisl for Staphylococcus Aureus Indian Journal of Applied Research, Vol.4, Issue.7 July 2014

Number of Downloads : 490

References :