Volume : IV, Issue : XII, December - 2014

Cytotoxic investigation of stem bark extracts of Terminalia chebula Retz.

Iwin C. Varkey, G. Mangai Kasthuri

Abstract :

<p>Terminalia chebula a native plant in India and Southeast Asia commonly known as Haritaki has been reported to exhibit a variety of biological activities. The present study aimed to investigate the cytotoxicity of the stem bark extracts of Terminalia chebula Retz. Methanol, chloroform, petroleum ether and aqueous extracts< /> were used for the study. Percentage cell viability of cell lines was carried out by using trypan blue dye exclusion technique. The viability percentage of cell lines analysed after exposing the cells to various solvent extracts showed difference in cell tolerating ability. These extract was screened for its cytotoxicity against HeLa and cell lines at different< /> concentrations to determine the growth inhibition by MTT assay. It was found that the % growth inhibition increased< /> with increasing concentration steadily upto 100mg/ml on HeLa cell line. Methanolic extracts shows higher cytotoxicity< /> at a concentration of 100mg/ml. The DNA fragmenting ability of bark methanolic extract is effective when compared< /> to other solvent extracts. This was mainly due to the presence of quinones and phenolic compounds in the methanol< /> extract. Chloroform and petroleum extracts showed less DNA fragmentation due to the absence of these metabolites.< /> It was followed by acute oral toxicity studies. The bark extract of T. chebula when administered for analysing the acute< /> oral toxicity test showed no deleterious effects were observed in the experimental animals.</p>

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Iwin C. Varkey,G. Mangai Kasthuri Cytotoxic investigation of stem bark extracts of Terminalia chebula Retz. Indian Journal of Applied Research, Vol.4, Issue : 12 December 2014

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