DARK FERMENTATIVE HYDROGEN PRODUCTION FROM BREWERY EFFLUENT USING THREE PURE ISOLATES

B. Satish kumar; N. Chaitanya; V. Himabindu; M. Lakshmi Narasu

Volume : VII, Issue : III, March - 2017

DARK FERMENTATIVE HYDROGEN PRODUCTION FROM BREWERY EFFLUENT USING THREE PURE ISOLATES

B. Satish Kumar, N. Chaitanya, V. Himabindu, M. Lakshmi Narasu

Abstract :

Brewery industries release a large amount of effluent with high COD suitable as a source of substrate for hydrogen production. Organisms isolated from anaerobic sewage sludge, which was found to be a suitable mixed culture for hydrogen production in previous studies, were used in this study. 16S RNA analysis shown that the three organisms isolated were Bacillus subtilis, Enterococcus faecalis and Acinetobacter calcoaceticus. Present studies were conducted in 320 ml batch reactors with a working volume of 250 ml at the pH 6.5. Among the three pure organisms studied, Bacillus subtilis has shown a highest H2 yield of 378 ml/ l effluent after 42 hr at a rate of 9 ml/l/hr. In the volatile fatty acid analysis, it was found that Bacillus subtilis and Enterococcus faecalis were producing acetic acid majorly, whereas, Acinetobacter calcoaceticus produced butyric acid.