Volume : VIII, Issue : II, February - 2018

Detection of Biofilm and Beta Lactamases Production in Clinical Isolates of Pseudomonas aeruginosa.

Neena V. Nagdeo, Suchita Netam

Abstract :

 

Pseudomonas infections are difficult to eradicate because of their intrinsic resistance as well as their capacity to acquire resistance to different antibiotics. MBL producing isolates are associated with higher morbidity and mortality. Biofilm production is an inherent ability of bacteria which can lead to enhanced virulence .

A total of 71 Pseudomonas aeruginosa were isolated from clinical samples like pus, urine, sputum, catheter tip etc.All the isolates were tested for ESBL and AmpC production by Camilla Rodrigues disk placement method. Modified hodge test was used for detection of MBL producing isolates of pseudomonas. Biofilm production was detected by tube method as mentioned by Basu et al.

Out of 71 pseudomonas aeruginosa isolated from various clinical samples, 92% (n=65) are biofilm producer. MBL production in our study was 5% . our study indicates that P. aeruginosa is becoming resistant to commonly used antibiotics and mostly biofilm producers. These strains should be routinely tested for ESBL production for better patient care.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Neena V. Nagdeo, Suchita Netam, Detection of Biofilm and Beta Lactamases Production in Clinical Isolates of Pseudomonas aeruginosa., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-8 | Issue-2 | February-2018

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