DETECTION OF BIOFILM BY THREE DIFFERENT METHODS IN VIRULENT STRAINS OF CLINICALLY SIGNIFICANT COAGULASE NEGATIVE STAPHYLOCOCCI

DR.S.DEEPA; DR.B.PADMINI

Volume : VII, Issue : V, May - 2017

DETECTION OF BIOFILM BY THREE DIFFERENT METHODS IN VIRULENT STRAINS OF CLINICALLY SIGNIFICANT COAGULASE NEGATIVE STAPHYLOCOCCI

Dr. S. Deepa, Dr. B. Padmini

Abstract :

Coagulase Negative Staphylococci( CoNS) are ubiquitious micro organisms. CoNS previously dismissed as contaminants are now emerging as important potential pathogens. The pathogenicity of this clinically significant CoNS strains is due to the production of biofilm (slime). The slime permits these microorganisms to adhere on implants and defend themselves from host immune system and antimicrobial therapy. Ninty clinically significant CoNS isolates were included in this study. Isolates were speciated by standard microbiological methods . Biofilm detection was done by Micro titre plate method , Tube Method, and Congo red Agar method. . Of the 90 isolates tested by MTP method for biofilm formation, 13(14%) were strong biofilm producers and 34 (37.7%) were moderate biofilm producers. When the three methods were compared Micro titre plate method was most reliable and easy method so it can be used as a General screening method for detection of Biofilm producing Bacteria