Detection of Biofilm Formation Among the Clinical Isolates of Staphylococcus Aureus

K. Bhaskaran; M.J. Haja Abdul Nazeer; K. Sethumadhavan

Volume : V, Issue : IV, April - 2015

Detection of Biofilm Formation Among the Clinical Isolates of Staphylococcus Aureus

K. Bhaskaran, M. J. Haja Abdul Nazeer, K. Sethumadhavan

Abstract :

BACKGROUND: Biofilms cause significant problems in the environment and during the treatment of infections. Biofilm–associated infections are characteristically chronic and frequently occur in hospitals.

Staphylococcus aureusis a leading cause of such infections [1].

OBJECTIVE: The objective of this study was to detect the biofilm–forming ability of Clinical isolates of S. aureus in our

Hospital.Material andMethods:Among 90 Staphylococcus spp., only 45 (50.00%) strains were identified as S. aureus

by standard conventional microbiological methods. These strains were screened by Tissue Culture Plate (TCP), & Tube

method (TM) for detection of biofilm formation.Results: Of 45 (50.00 %) strains of S. aureus, 01 (20.00 %) in Blood

samples, 2 (20.00 %) in Pus, 03(42.85 %) in Urine, 02 (33.33 %) in Sputum, 05 (50.00%) in Urinary catheter tips &06

(85.71%) in Central venous catheters. Strong biofilm production was detected in TCP method 11 (57.89%) as while

Moderate biofilm producers by TM method detected is 08 (42.10%).Antibiotic susceptibility testof biofilm producing

bacteria was performed by using the Kirby–Bauer disc diffusion technique,&higher antibiotic resistance was observed

in biofilm producing bacteria than non biofilm producers. Conclusion: We can conclude from our study that the TCP

method was found to be most Sensitive, accurate &reliable method for the detection of biofilm forming microorganisms as compared to TM, and it can be recommended as a general screening method for detection of biofilm producing bacteria in laboratories.