DETECTION OF BIOFILM PRODUCING STAPHYLOCOCCI AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN FROM PUS SAMPLES IN A TERTIARY HEALTH CARE CENTRE

Dr. Astha; Dr. Ruchi Rati; Dr. Suman Rishi

Volume : VI, Issue : III, March - 2016

DETECTION OF BIOFILM PRODUCING STAPHYLOCOCCI AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN FROM PUS SAMPLES IN A TERTIARY HEALTH CARE CENTRE

Dr. Astha, Dr. Ruchi Rati, Dr. Suman Rishi

Abstract :

 Biofilms are microbial communities of the surface–attached cells, embedded in a self–produced extracellular polymeric matrix Aim: To detect the biofilm producing Staphylococci from pus samples and compare the different methods used for biofilm detection. Methods & Results: In the present study, three different methods for detection of biofilm formation were used and their results were compared to find out the most appropriate method for demonstrating biofilm. Out of the 78 isolates, the Tissue Culture Plate (TCP) method detected biofilm in 41 isolates (52.56%), Tube Method (TM) detected biofilm in 32 isolates (41.03%) and Congo Red Agar (CRA) method detected biofilm in 4 isolates (5.13%). The present study showed the TCP method to be most sensitive for the biofilm detection, followed by TM and CRA method.