DETECTION OF EXTENDED SPECTRUM BETALACTAMASES AND THEIR ANTIBIOTIC SUSCEPTIBILITY AMONG URINARY ISOLATES

Dr N Mythily; Dr V Vijayashree; Dr T Ravikumar; Dr P Malini

Volume : VII, Issue : III, March - 2017

DETECTION OF EXTENDED SPECTRUM BETALACTAMASES AND THEIR ANTIBIOTIC SUSCEPTIBILITY AMONG URINARY ISOLATES

Dr N Mythily, Dr V Vijayashree, Dr T Ravikumar, Dr P Malini

Abstract :

Background : Urinary tract infections (UTI) are one of the most common bacterial infections in humans worldwide. A study on the changing antibiotic sensitivity pattern is pertinent for an appropriate treatment and for prevention and control of the various mechanisms of drug resistance. Aims& Objectives: To perform antibiotic sensitivity of urinary isolates and to detect the extended spectrum betalactamase (ESBL) production among Escherichia coli, Klebsiella pneumoniae and Proteus by double disc synergy test (DDST) and Etest. Materials & Methods : A total of 422 samples collected during a period of six months were processed in blood agar and MacConkey agar . Antibiotic susceptibility tests done and ESBL isolates were detected using double disc synergy test and E test. Results : Escherichia coli was the most common isolate and also an ESBL producer (67.5%). ESBL isolates are more susceptible to Meropenem, Nitrofurantoin, and Amikacin. Conclusion : E.coli was the predominant ESBL producer . Coresistance of non beta lactum antibiotics was also observed.