Detection of Human cytomegalovirus (HCMV) Epstein Barr virus (EBV) and Herpes simplex virus (HSV) in periodontal disease and effect of scaling and root planing (SRP) on the presence of these viruses.

Bharati  Kolliyavar; Swati  Setty; Srinath L.Thakur

Volume : IV, Issue : XII, December - 2014

Detection of Human cytomegalovirus (HCMV) Epstein Barr virus (EBV) and Herpes simplex virus (HSV) in periodontal disease and effect of scaling and root planing (SRP) on the presence of these viruses.

Bharati Kolliyavar, Swati Setty, Srinath L. Thakur

Abstract :

Aim: To evaluate the presence of human cytomegalovirus (HCMV), Epstein– Barr virus (EBV) and herpes simplex virus type 1 (HSV–1) in subjects with chronic periodontitis and periodontally healthy subjects and effect of scaling and root planing (SRP) on the presence of these viruses. Materials and methods: At baseline in the test group, subgingival plaque samples were collected from the periodontal pocket and in the control group, plaque samples were collected from the gingival sulcus. In the test group after the baseline plaque sampling was done, scaling and root planing was performed. Plaque was collected after 8 weeks of the SRP again. Hot STAURT multiplex PCR method was used to detect the viruses in the plaque sample. Results: Overall the presence of HSV–1 in the test group was statistically high at base line compared to the control group. There was reduction in HSV–1, HCMV and EBV after SRP in the test group compared to the baseline. Conclusion: Herpes viruses were found in higher frequency in chronic periodontitis patients than in healthy subjects. SRP reduced the percentage of these viruses after 8 weeks.