Detection of Staphylococcus aureus and Methicillin–Resistant Staphylococcus aureus  (MRSA) from Human Clinical Specimens Using Conventional Biochemical Tests and Chromogenic Media

Abdulameer Abdullah Al-Mussawi

Volume : IV, Issue : II, February - 2014

Detection of Staphylococcus aureus and Methicillin–Resistant Staphylococcus aureus (MRSA) from Human Clinical Specimens Using Conventional Biochemical Tests and Chromogenic Media

Abdulameer Abdullah Al Mussawi

Abstract :

Objective: To identify of Staphylococcus aureus and methicillin - resistant staphylococcus aureus (MRSA) by two methods; conventional biochemical test and chromogenic media from various clinical specimens in Basrah, Iraq.

Methods: A total of 82 isolates from clinical specimens were collected ( wounds , skin infections, urine samples and stool samples) . All these isolates were tested for comparative detection S. aureus by conventional biochemical test and by CHROMagar Staph. aureus , all positive samples were detected MRSA by CHROMagar MRSA.

Results: 26 (31.7%) isolates of 82 clinical samples were detected as S. aureus by conventional biochemical test, while 23 (28%) were positive by CHROMagar Staph. aureus. 17 (65%) from 26 isolates as MRSA detected by CHROMagar MRSA.

Conclusion: A screening procedure using chromogenic media give better results, faster and more sensitive in identifying S. aureus and MRSA than conventional methods.