Volume : V, Issue : VI, June - 2015

Detection the AI dead sperms via florescent microscope with the investigation of DNA fragmentation by electrophoresis

Anmar Jasim Mohammed, Saad Akram Hatif

Abstract :

 This study included the evaluation of semen samples in the artificial insemination center of Iraqi. Ejaculated semen was collected from thirteen mature Holstein bulls by artificial vagina , semen samples subjected to examination in the deferent stages of preparation , like collection , dilution , cooling and freezing point . Sperms stained by florescent dye ( ethidium omide) subsequent investigation of the sperm viability via florescent microscope , in addition the investigation of mid piece defect . Molecular inspection inclusive the genomic DNA extraction by ( phenol – chloroform method ) , total DNA product running in the agarose gel by electrophoresis to detect the DNA fragmentation . The feature of DNA fragmentation reinforce with the result of sperm colored (orange– reddish) of sperm head and , another defect in the midpiece , like swelling , thin , irregular , absent, and short .In conclusion , the manipulation of the AI semen preparation lead to decline sperms quality , increase sperms dead , and DNA fragmentation.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Anmar Jasim Mohammed, Saad Akram Hatif Detection the AI dead sperms via florescent microscope with the investigation of DNA fragmentation by electrophoresis Indian Journal of Applied Research, Vol.5, Issue : 6 June 2015

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