DEVELOPMENT OF CHICK EMBRYO DIABETES MODEL FOR SCREENING OF ANTIDIABETIC DRUGS

P. Kranthi Raju; K. Thirupathi; Shravan kumar Dholi; N. Prasad

Volume : IX, Issue : VIII, August - 2019

DEVELOPMENT OF CHICK EMBRYO DIABETES MODEL FOR SCREENING OF ANTIDIABETIC DRUGS

P. Kranthi Raju, K. Thirupathi, Shravan Kumar Dholi, N. Prasad

Abstract :

The aim of the present study was to investigate whether young chickens with diabetes could be developed by treating the chick during emyonic stage with streptozotocin (STZ) or alloxan. The model was validated for testing antihyperglycemic activity using a standard drug, glipizide. Hatched eggs were divided in to three groups. On the day 14 of incubation, holes were made on the shells of the eggs by using driller, and then were injected with STZ [0.3mg/kg] and alloxan.Control group kept with out any treatment [n=8 in each group]. The holes were closed using tape and the eggs were incubated for another 7days (up 21st day). Chicks that would came out were kept in specially made cages .After two weeks ,blood glucose levels were estimated using chemstrip method using Glucometer.Hyperglycemic chicks were separated and treated with standard drug glipizide[0.5mg/kg],and the blood glucose levels were estimated at different time intervals [30, 60, 120 min].The results were analyzed statistically by ANOVA. The levels of blood glucose significantly [STZ& Alloxan: **P <0.01] increased in STZ/alloxan treated groups as compared to the control and there was a significant [STZ: *P <0.05; Alloxan **P<0.01] decrease after treating with standard drug within 30–60min. The results of the present study indicate that STZ, alloxan– if administered during emyonic stage would result in a stable diabetic chicks after hatching and this model can be used to screen drugs for anti–diabetic activity.