Direct in vitro Regeneration of Arachis Hypogaea  L.(Jl–24) and Study of Salt Tolerance

Bhagya Lakshmi Jyothi Kusuma; Sharmila Begum S

Volume : III, Issue : VIII, August - 2013

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl–24) and Study of Salt Tolerance

Bhagya Lakshmi Jyothi Kusuma, Sharmila Begum S

Abstract :

An efficient protocol for complete in vitro plant regeneration of Arachis hypogea Linn (.JL–24) variety is devised in the present study. Immature emyos when cultured on MS1 regeneration media (MS + 2.5 mg/L BAP + 0.2mg/l NAA) has shown a higher frequency of regeneration. There are no such reports of direct regeneration using emyo culture. Efforts have been made to develop salt stress tolerant plants using this protocol by subjecting in vitro developed plant to different NaCl regimen (concentration) in MS2(MS1+ NaCl) medium. The regenerated plants survived and were found to be tolerant to a maximum concentration of 1.0mg/L NaCl