Direct shoot organogenesis from bulbs explants of  Polianthes tuberosa  cultivars (Prajwal and Shringar)

Jitendriya Panigrahi; Nikita M Mistry; Illa C Patel

Volume : III, Issue : VII, July - 2013

Direct shoot organogenesis from bulbs explants of Polianthes tuberosa cultivars (Prajwal and Shringar)

Jitendriya Panigrahi, Nikita M Mistry, Illa C Patel

Abstract :

Ornamental plant Polianthes tuberosa (Amaryllidaceae) contains flowers which are used in wedding ceremonies, garlands, decoration and various traditional rituals. The present investigation given emphasis on: 1. In vitro propagation of two hyid variety of Polianthes tuberosa namely Prajwal and Sringar from bulb as explant. 2. Effect of different combinations of BAP and Kinetin for its shoot proliferation, and to standardize the optimum concentration of growth regulators for proper growth of these hyid varieties of Polianthes tuberosa. The rate of shoot proliferation frequency was gradually raised and reached maximum at 2.0mg/l concentration of both BAP and Kn in Prajwal as compared to 0.2mg/l in Shringar. The plantlets were incubated in a solution of IBA (0.5 mg l–1) and IAA (2.0 mg l–1) and IBA (0.5mgl–1) and IAA (2.5 mgl–1) at 50 C to induce rooting of Prajwal and Shringar varieties respectively