Volume : I, Issue : XII, September - 2012

Dna Polymorphism Studies On Ghrelin Gene In Chicken

M Ekhtiari Sadegh, C S Nagaraja

Abstract :

Ghrelin is a key actor in the hypothalamic melanocortin system. The present investigation was carried out to study nucleotide sequencing and DNA polymorphism by PCR–RFLP of ghrelin gene in four strains of chicken. Genomic DNA was isolated from a total of 200 birds belonging to four Indian strains of chicken namely new Genotype, Punjab Broiler, Indian Cornish and University Male Line. A fragment of ghrelin gene, comprising of a partial of intron 3, complete exon 4 and partial intron 4, was amplified. The products from each variety were digested with Hinf I, which recognizes the 5– G^ANTC–3 sequence. The RFLP pattern revealed three genotypes (LL, VV and GG) and three different alleles (L, V and G).  

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

M Ekhtiari Sadegh, C S Nagaraja Dna Polymorphism Studies On Ghrelin Gene In Chicken Indian Journal of Applied Research, Vol.I, Issue.XII September 2012

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