Drug susceptibility pattern of Mycobacterium tuberculosis in patients with pulmonary tuberculosis.

Dr. Rajesh Kumar Jain .S.; Dr. Paramjyothi .G. K.

Volume : V, Issue : XI, November - 2015

Drug susceptibility pattern of Mycobacterium tuberculosis in patients with pulmonary tuberculosis.

Dr. Rajesh Kumar Jain . S. , Dr. Paramjyothi . G. K.

Abstract :

 BACKGROUND: The literature regarding drug resistant tuberculosis (DRTB) is scanty in most of the regions of the country. Hence the present study was undertaken to understand the drug susceptibility pattern of Mycobacterium tuberculosis (MTB) in patients with pulmonary tuberculosis (PTB). MATERIALS AND METHODS: Early morning satisfactory sputum sample was collected and subjected to sputum smear microscopy after Ziel–Neelsen (ZN) staining as per Revised National Tuberculosis Control Programme (RNTCP) guidelines. All the sputum samples were delivered by courier in a cold chain to Dr. Iravatham’s Clinical Laboratory, Hyderabad and were processed using the N–acetyl–L–cysteine – sodium citrate – sodium hydroxide method. Sediment was subjected to ZN staining, inoculated on Lowenstein–Jensen media for 8 weeks and Line Probe Assay (LPA) (according to the manufacturer’s instructions (Genotype MTBDRplus, Hain Life–Science, Nehren, Germany). RESULTS: Out of 67 cases enrolled in our study, resistance to Isoniazid (H) is seen in 4 cases (5.97%), resistance to Rifampicin (R) in 18 cases (26.86%), resistance to both H and R in 22 cases (32.83%). The sensitivity to both H and R is seen in 20 cases (29.85%). CONCLUSION: Rate of drug resistance PTB is high in patients been on irregular treatment and patients previously treated.