Effect of Explant Type and Different Plant Growth Regulators on Callus Induction and Plantlet Regeneration in Grapefruit, Citrus decumana var. paradisi (Macfad.) H. H. A. Nicholls

Dr. Babita Rana; Dr. Harshad M. Pandit

Volume : V, Issue : VII, July - 2015

Effect of Explant Type and Different Plant Growth Regulators on Callus Induction and Plantlet Regeneration in Grapefruit, Citrus decumana var. paradisi (Macfad.) H. H. A. Nicholls

Dr. Babita Rana, Dr. Harshad M. Pandit

Abstract :

<p>In vitro propagation of grapefruit using various explants results in rapid and efficient propagation, pathogen free plants, increased fruit yield and enhanced metabolic production useful to industry.The present investigation was carried out to evaluate the effect of types of explant, growth medium and growth regulators on callus induction and subsequent regeneration of grapefruit plant using direct and indirect organogenesis method. The nodal explants obtained from in vitro grown seedling cultured on Murashige and Tucker (MT) medium supplemented with Malt extract (0.5 gm/L) + kinetin (0.5 mg/L) + NAA (5mg/L) showed 88.70 percent of callus induction. The epicotyl segments cultured on MT medium containing 3 mg/l BAP, 0.5 mg/l NAA and malt extract @ 0.5 mg/l proved to be best explants for shoot bud induction with an average of 7.8 buds per explant. On the other hand for indirect organogenesis the callus derived from nodal tissue cultured on MT medium containing 1mg/l BAP, 0.5 mg/l NAA and malt extract @ 0.5 mg/l performed well for shoot bud induction. Regenerated shoots were rooted on half strength MT medium containing IBA (1 mg/l).</p>