Volume : IV, Issue : III, March - 2014

Effect of Irradiation on Genotoxicity of Two Resin Modified Glass Ionomer Luting Cements – An in–Vitro Study

Mithra N. Hegde, Shilpa . S. Shetty, Shabin. S, Nidarsh D. Hegde, Suchetha Kumari, Ganesh Sanjeev

Abstract :

The aim of the study was to evaluate the genotoxicity before and after electron beam irradiation of two commercially available  resin modified glass ionomer luting cements –Rely X Luting and Rely X Luting plus on human lymphocytes invitro. Samples were prepared according to ISO standards and divided into two groups– Non radiated and radiated groups. The radiated group was exposed to 200Gy of  electron beam irradiation. The sealer extraction was done for a  duration of 24 hours in DMEM media. Lymphocyte was separated and used for genotoxicity study. Statistical analysis was performed using student‘t’ test. The irradiation of Rely X luting cement with 200Gy dose of electron beam irradiation showed increase in the frequency of DNA damage when compared to that of the non–radiated group. Similar results were also seen in RelyX Luting Plus cement with 200Gy dose. Apoptotic diffusion also showed a significant increase in DNA diffusion in lymphocytes that were incubated with irradiated materials of Rely X Luting and Rely X  Luting Plus  . Statistical significance was observed in Relyx Luting  Plus (p=0.02). Within the limitations of the present study, it is concluded that further research is needed to increase the polymerization of the resin based glass ionomer luting cements and decrease the genotoxicity by pretreatment of the material.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Mithra N. Hegde, Shilpa .S.Shetty, Shabin.S, Nidarsh D.Hegde, Suchetha Kumari, Ganesh Sanjeev Effect of Irradiation on Genotoxicity ofTwoResinModified Glass Ionomer Luting Cements‾An in-Vitro Study Indian Journal of Applied Research, Vol.IV, Issue. III

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