EFFECT OF PHYTOCHEMICAL KAEMPFEROL ON HCT–15 AND LYMPHOCYTES

CHEPURI KALYANI; MANGAMOORI LAKSHMI NARASU; YUMNUM PRIYADARSHINI DEVI

Volume : V, Issue : X, October - 2015

EFFECT OF PHYTOCHEMICAL KAEMPFEROL ON HCT–15 AND LYMPHOCYTES

Chepuri Kalyani, Mangamoori Lakshmi Narasu, Yumnum Priyadarshini Devi

Abstract :

Kaempferol is a flavonoid found abundantly in many plants. Many investigations revealed its anti–microbial, anti–cancer, cardio protective, anti–oxidant activities. In the present study, we screened the effect of kaempferol on human colon cancer cell line (HCT–15) and human normal lymphocytes at different concentrations. The morphological alterations were observed using microscopy and the cytotoxicity was evaluated by MTT assay. Kaempferol showed dose dependent antiproliferative activity on HCT–15 after 24hrs of incubation. The study was compared with chemotherapeutic drugs Doxorubicin and Cisplatin. The IC50 concentrations were found to be 120 μg/ml , 50 ug/ ml and 25 ug/ml for Kaempferol, Doxorubucin and Cisplatin respectively. However, normal human lymphocytes were not affected with Kaempfero