Volume : V, Issue : II, February - 2015

Effect of UV irradiation and Colchicine on Cellulase enzyme production by Aspergillus terreus

Johra Khan, Rizvi Moattar Raza, Manab Deka

Abstract :

Development of more efficient cellulases having higher activities and stability to enable the conversion of pretreated biomass to higher yield at lower costs will significantly reduce the process costs. Cellulase are multi–enzymatic complex protein comprising of endo–1,4–beta–D–glucosidase, or endocellulase, carboxy methyl cellulase(CX); Exo–1,4,β–D–glucosidase or beta–glucosidase. The enzymatic saccharification of cellulase has been proposed as a means of producing glucose with the objective of reconstructing cellulase to enhance biomass degradation on an industrial scale. Wild stain of Aspergillus terreus was isolated from agriculture waste and selected to mutagenesis with ultra violet (UV) irradiation and Colchicine. In UV–irradiated Aspergillus terreus (At1) the production of CX increased by 24–folds, β–glucosidase production increased by 55–folds and 84–fold increase in C1 (Cotton Assay) production in 60 minute exposure time. Colchicine treatment 0.1% (W/V) increased the production of CX to 81–folds, β–glucosidase production increased by 93–folds and 95–fold increase in C1 production with same species and in same duration.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Johra khan,Rizvi Moattar Raza,Manab Deka Effect of UV irradiation and Colchicine on Cellulase enzyme production by Aspergillus terreus Indian Journal of Applied Research, Vol.5, Issue : 2 February 2015

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