EFFECT OF VAGINAL CANDIDIASIS AND TREATMENTS WITH OILS OF THYME AND OREGANO COMPARED WITH NYSTATIN ON BLOOD PARAMETERS OF MATURE FEMALE RABBITS

Bushra F. Hasan   Abdulrazak N. Khudair    Jassim M. A. Alkalby

Volume : V, Issue : XI, November - 2015

EFFECT OF VAGINAL CANDIDIASIS AND TREATMENTS WITH OILS OF THYME AND OREGANO COMPARED WITH NYSTATIN ON BLOOD PARAMETERS OF MATURE FEMALE RABBITS

Bushra F. Hasan Abdulrazak N. Khudair Jassim M. A. Alkalby

Abstract :

 Oils of thyme and oregano were extracted by hexane and analyzed to the component in a manner GC–MAS (Gas chromatography). In vitro study was conducted to find the best oily concentration capable of inhibiting growth of candida albicans culture on sabaroud medium. in vivo study was done on seventy two healthy mature non pregnant female rabbits. Animals were divided into nine groups of eight animals each. The first group was injected by (0.1ml) physiological saline vaginally and served as a negative control group. All other eight groups were immune suppressed by drenching each animal with (5 mg/kg) dexamethasone once daily for 7 days,(Group 2) considered as a dexamethasone control group. Other seven groups were infected vaginally with Candida albicans by (1.5)×?10?^8 candida cells/ml once daily for 7 days (Group 3) considered as vaginal candida (positive) control. The sixth groups then treated once daily for 14 days with (0.1) ml of oils according to each group. (Group 4) was treated with thyme oil orally, (Group5) treated with thyme oil vaginally ,(Group 6) treated with oregano oil orally,(Group 7) treated with oregano oil vaginally,(Group8)treated oral nystatn,(Group9) treated vaginally with nystatin. Blood was drawn from animals at the end of the experiment. Result conducted to experimental vaginal candidiasis(EVC) did not has any effect on RBCs so as treatment with thyme and oregano oils while RBCs significantly decreased with treatment nystatin by two routes. Hb decreased with (EVC) and only treatment with vaginal thyme oil raised Hb to normal value. PCV did not change in all groups of study. Total WBCs decreased in (EVC) and all treatment groups did not improve this effect except oral thyme oil treatment. Increased lymphocytes in (EVC), thyme oil and oregano oil orally than control. significant decrease in granulocyte in all studies groups.(EVC) did not affect platelets count however oral(thyme or oregano)oil and vaginal nystatin caused decreased in platelets count contrary to the increase platelets in vaginal (thyme or oregano)oil and oral nystatin. MCV increased in vaginal nystatin and decreased in oral nystatin while MCHC increased in vaginal thyme and decrease in vaginal nystatin