Effects of Flavonoids From Andrographis Serpyllifolia Leaves Extract on Carbon Tetrachloride–Induced Hepatotoxicity in Wistar Rats

Shyam Sundar Gupta; Joni Sharma; Ch. V. Rao

Volume : IV, Issue : V, May - 2014

Effects of Flavonoids From Andrographis Serpyllifolia Leaves Extract on Carbon Tetrachloride–Induced Hepatotoxicity in Wistar Rats

Shyam Sundar Gupta, Joni Sharma, Ch. V. Rao

Abstract :

The current study investigated the effect of standardized extract of Andrographis serpyllifolia leaves on car – bon tetrachloride–induced hepatotoxicity in Wistar rats. Hepatoprotective activity was analyzed by biochemical parameters like serum enzymes, Aspartate transaminase (AST), Alanine transaminase (ALT), Serum Alkaline Phosphatase (ALP) and total serum bilirubin (T.Bil) and antioxidant parameters like Lipid peroxidation (LPO), Superoxide dismutase (SOD), Catalase (CAT), and Reduced glutathione (GSH) against CCl4 induced hepatotoxicity. Treatment with standardized extract at dose level of 125, 250 and 500 mg/kg showed 19.84–47.74%, 27.84–55.86%, and 52.20–74.65% protection respectively with the depletion of AST, ALT ALP and T.Bil in serum, significantly reduced the lipid peroxidation (from 0.62±0.07 to 0.40±0.09) and SOD (from 204.2±13.1 to 101.2±0.8) and increased in levels of catalase (from 30.2±1.2 to 35.4±1.3) and GSH (from 40.5±2.8 to 59.6±3.7). Based on AST, ALT, ALP, Total bilirubin levels and antioxidant estimation, the ethanol extract of Andrographis serpyllifolia showed antihepatotoxic effect as compared to silymarin.   