Volume : V, Issue : VIII, August - 2015

EFFICIENT ROOTING AND BIOLOGICAL HARDENING OF IN VITRO PLANTLETS OF RUTA GRAVEOLENS L.

Ajit A. Kengar, Govind. T. Paratkar

Abstract :

 T In the present paper, an efficient protocol for rooting and acclimatization of invitro plantlets raised from direct and indirect organonogenesis cultures of Ruta graveolens L.is described.The regenerated shoots from direct and indirect organogenesis plants were excised and transferred to half strength MS medium with different concentrations of IBA and NAA.The better response of rooting was observed in plants cultured with IBA. At the concentration IBA (2.46µM) frequency of root formation was better than other concentration of IBA. 90% shoots were developed on an average 4.5 ± 0.50 roots.The rootedplantlets were grown in pots containing different hardening media.The hardened plants were analyzed in terms of survival rate, length of shoot and morphology The hardening media soil: sand: compost 1: 1: 1 showed 90% survival rate with 12–13cm shoot length having erect stem. The hardened plantlets were successfully acclimatized in the greenhouse and transferred to open field conditions

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Ajit A. Kengar, Govind.T. Paratkar Efficient Rooting and Biological Hardening of In Vitro Plantlets of Ruta Graveolens L. Indian Journal of Applied Research, Vol.5, Issue : 8 August 2015

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