Volume : I, Issue : VI, March - 2012

Encapsulation of Lactic Acid Bacteria in Calcium Alginate Beads for Bacteriocin Production

Vijay Wadhai, Shweta Kayarkar, Archana Dixit

Abstract :

In the present study, encapsulation of lactic acid bacteria in alginate beads were carried out. For this purpose a bacteriocin producing strain Lactobacillus bulgaricus was used. To enhance of the capsule stability, batch fermentations was performed in MRS medium without phosphates and with addition of CaCl2. As a result of the immobilization ,the bacteriocin production was increased in encapsulated cells as compared to free cells. Well diffusion method was used for the detection of antimicrobial activity. The bacteriocin produced by Lactobacillus bulgaricus exhibited the zone of inhibition against Staphylococcus aureus. As compared with the free cells encapsulated cells shows the maximum bacteriocin activity. In the purification of bacteriocin, filtrate culture, was removed by centrifugation, and the protein were concentrated by 80% ammonium sulfate precipitation and dialysis. The recovered proteins were then fractionated by ion–exchange chromatography

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Vijay Wadhai, Shweta Kayarkar, Archana Dixit Encapsulation of Lactic Acid Bacteria in Calcium Alginate Beads for Bacteriocin Production Indian Journal of Applied Research, Vol.I, Issue.VI March 2012

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