Enhancement of cellulase production by substrate manipulation in three Aspergillus sp.

Divyani Agrawal; Dr. Ketna Matkar

Volume : VI, Issue : III, March - 2016

Enhancement of cellulase production by substrate manipulation in three Aspergillus sp.

Divyani Agrawal, Dr. Ketna Matkar

Abstract :

 Aspergillus fumigatus, Aspergillus ellipticus and Aspergillus fumigatus Fersenius were employed for production of cellulase under solid state fermentation conditions using 10 mesh size wheat straw. There was significant increase in cellulase production upon pretreatment of substrate using a combination of 0.75% (v/w) H2SO4 and 1.5% (w/v) NaOH at 10% (v/v) loading for 90 min and 120 min respectively. There was a 1.18–fold increase in filter paper activity upon pretreatment which was further increased by 2.21–fold upon mesh size reduction. There was an overall increase of 4.76, 3.62 and 1.40 fold in exoglucanase, endoglucanase and β–glucosidase activity upon optimization. This proves that significant increase in cellulase efficiency can be achieved by manipulation of substrate.