Enhancing ethanol yield from sugar cane molasses fermentation by addition of depolymerising enzymes

Fadel; M; Abdel Nasser A. Zohri; Adel M..Kamal El�Dean; A.H. Auob; B. Deiab

Volume : VI, Issue : VIII, August - 2016

Enhancing ethanol yield from sugar cane molasses fermentation by addition of depolymerising enzymes

Fadel, M, Abdel Nasser A. Zohri, Adel M. . Kamal El Dean, A. H. Auob, B. Deiab

Abstract :

The demand of energy specially an eco–friendly clean biofuel is increased due to shortage of resources which leads to search for alternate fuel to replace fossil fuels.Sugarcane molasses (the by–product of sugar industry from sugarcane) is a main raw material for bioethanol production in many countries. The cost of molasses in increasing sothere is a need to improve the ethanol production from cane molasses. Molasses nearly involved about 10 % unfermentable sugars relative to the fermentable sugars. as alpha amylase, glucoamylase, dextranase and cellulase were applied to eak down the biopolymer such as starch,dextran and cellulose to monosacchadides can be fermented to ethanol by Saccharomyces cerevisiaeduring the process of fermentation. Enzymes level addition of alpha amylase, glucoamylase,dextranase and cellulase to fermentation molasses medium were optimized to be 4,6,3 and 2 mg /100g unfermentable sugars, respectively.The fermentation applied in sugar cane molasses Brix 21 contained 16.6 % total sugars involved 1.8 % unfermentable sugars. The fermentation was carried out for 36 hrs at 35oC by S. cerevisiae F–514 giving ethanol yield 8.89% v/v compared to 8.17% v/v in untreated enzyme molasses.The obtained ethanol yield increase resample 8.81% v/v.