Enzymological Responses in Channa punctatus Exposed to Sub–lethal Concentration of Hexaconazole (Contaf) in Paddy–cum–fish Ecosystem

Neelanjana Choudhury; Jayanta Tarafdar; Ashis Panigrahi

Volume : V, Issue : II, February - 2015

Enzymological Responses in Channa punctatus Exposed to Sub–lethal Concentration of Hexaconazole (Contaf) in Paddy–cum–fish Ecosystem

Neelanjana Choudhury, Jayanta Tarafdar, Ashis Panigrahi

Abstract :

Pesticides usage in agricultural fields to control pests is extremely toxic to non target organisms like fish and affect fish health through impairment of metabolism, sometimes leading to mortality. Present study is an attempt of investigating enzymological responses in Channa puntatus exposed to fungicide Hexaconazole used against Rhizoctonia solani in rice plants in paddy–cum–fish ecosystem. Native polyacrylamide gel electrophoresis have been used to analyze malate dehydrogenase (MDH), lactate dehydrogenase (LDH) and peroxidase (Pox) isozymes in different tissues like gill, heart, liver, kidney, muscle and spleen of the Channa punctatus in order to study the tissue specificity of these isozymes. LDH and MDH isozymes are major system found in liver and kidney and associated with environmental stressors gets elevated. Peroxidase, is related to antioxidant activity against free radical toxicity induced by fungicides, was found highly expressed in gill and heart. Lactate and malate dehydrogenase showed increased activity in spleen and kidney indicating detoxification and tissue damage induced by pesticide.