Evaluation of an Enzymatic Method for determining Serum Creatinine Assay

Sridhar K; Narayanan M; Mookherjee S; Goswami K

Volume : VI, Issue : VIII, August - 2016

Evaluation of an Enzymatic Method for determining Serum Creatinine Assay

Sridhar K, Narayanan M, Mookherjee S, Goswami K

Abstract :

The enzymatic method for the determination of serum creatinine is accepted as one of the standard method in a clinical laboratory. The enzymatic method for the determination of serum creatinine was optimized for use with Merilyzer AutoQuant–400 auto analyzer and its performance characteristics were practically compared with Jaffe’s Kinetic. Effects of some interfering substances like Serum bilirubin and plasma glucose on the Jaffe’s kinetic method and the enzymatic method were compared. We measured creatinine in serum samples with the enzymatic method and the Jaffe’s kinetic method in samples divided four groups; Group I–Samples without bilirubin and glucose; Group II–Sample with high level of plasma glucose ; Group III–Samples with high level of bilirubin and Group IV–all the samples. There was an excellent agreement between the two methods in terms of correlation coefficient even in the samples with high levels of glucose or bilirubin. Enzymatic method is having better sensitivity, less interfering effects & having better choice in making decision of critical management of the renal failure patient.