Evaluation of Antioxidant, Analgesic and Anti–Inflammatory Activity of Hydro – Alcoholic Extract and Ethyl Acetate Fraction of Withania Coagulans Dunal

Md.Akram Minhaj; Dr.S.R Mir; Prof.Mohd Ali; Md. Waris; Dr. Manoj Kumar; Ruquiah Khan

Volume : IV, Issue : V, May - 2014

Evaluation of Antioxidant, Analgesic and Anti–Inflammatory Activity of Hydro – Alcoholic Extract and Ethyl Acetate Fraction of Withania Coagulans Dunal

Md. Akram Minhaj, Dr. S. R Mir, Prof. Mohd Ali, Md. Waris, Dr. Manoj Kumar, Ruquiah Khan

Abstract :

Background: Present study is designed to evaluate antioxidant, analgesic and anti inflammatory potential of Withania coagulans Dunal in rat and mice which may be the basis for treating various challenging disorder. Objective: Evaluating antioxidant, analgesic and anti–inflammatory activity of Withania coagulans Dunal . Material and Method: Hydroalcoholic fraction and ethyl acetate fraction were extracted from fruits of Withania coagulans Dunal. Anti–inflammatory and analgesic activity was evaluated by carrageenan induced right hind paw edema and acetic acid induced writhing respectively. Antioxidant assay was performed by DPPH scavenging activity. Result: Hydro alcoholic fraction was found to be effective in treating inflammation (80.43 % inhibition as compared to diclofenac, 88.45 % ï‚± 0.35) and pain (72.78 % protection as compared to diclofenc, 87.00 %). Ethyl acetate fraction was also effective for treating inflammation (88.19% inhibition) and pain (86.32 % Protection). Both fraction acts as antioxidant (scavange DPPH). Conclusion: The anti–inflammatory and analgesic activity of ethyl extract of Withania coagulans are close to standard diclofenac with less ADR. Antioxidant potential can give additional benefit to the patients in free radical associated disorder