Evaluation of biological response of Radioresistant Bacterium INM–3 against the oxidative stress

Sushma; Poonam Malhotra; Raj Kumar; Shravan Kumar Singh

Volume : VIII, Issue : V, May - 2018

Evaluation of biological response of Radioresistant Bacterium INM–3 against the oxidative stress

Sushma, Poonam Malhotra, Raj Kumar, Shravan Kumar Singh

Abstract :

In the present study, RKIP006.G isolated from a radioresistant bacterium Bacillus sp. INM–1 was evaluated for its protection efficacy against of oxidative stress and a possible mechanism of resistance to radiation was studied for novel bacteria INM–3 isolated from rocky soil taken from QutubMeenar, Delhi in India. Isolation of bacteria INM–3 from soil, protection efficacy of RKIP006.G against hydrogen peroxide induced oxidative stress in bacteria INM–3, Radiation induced stress in bacteria INM–3, Protein estimation, and SDS–PAGE analysis methods were used to know the differential expressed protein which involved in protection activity of bacteria INM–3 in the presence of RKIP006.G.The results suggest that anti oxidative activity of bacteria INM–3 was induced by RKIP006.G and it was protected against oxidative stress induced by hydrogen peroxide. Further, observed that the isolated INM–3 bacteria have radio resistance characteristic against high dose of gamma radiation.