Evaluation of combinational treatment with quercetin and vitamin E against amphotericin B induced nephrotoxicity

S.Arulraja; S.Rajaram; G.Karthikeyan

Volume : IX, Issue : VII, July - 2019

Evaluation of combinational treatment with quercetin and vitamin E against amphotericin B induced nephrotoxicity

S. Arulraja, S. Rajaram, G. Karthikeyan

Abstract :

Free radical induced oxidative injury can play a key role in amphotericin B induced nephrotoxicity. Quercetin is strong free radicals trapper found in many plants and foods, for example apples, green tea, red wine, and onions. Vitamin E is a fat–soluble vitamin that exhibits significant role as an antioxidant. The present study was aimed to investigate the effect of quercetin and vitamin E against amphotericin B induced nephrotoxicity in rats. Nephrotoxicity was assessed by measuring various marker enzymes including Lactate dehydrogenase (LDH) Cathepsin–D, N–acetyl–β–D glucosaminidase, Alkaline phosphatase, Acid phosphatase, β–D–glucuronidase, Blood urea nitrogen, uric acid, creatinine and protein. The amphotericin B induced nephrotoxic animals exhibited elevation of γ–glutamyltranspeptidase Lactate dehydrogenase, Cathepsin–D, N–acetyl–β–D glucosaminidase, Alkaline phosphatase Acid phosphatase β–D–glucuronidase and lymphocytes levels whereas these enzyme levels were reverted back to near normal upon the administration of quercetin and vitamin E. the levels of urea, uric acid, creatinine and protein levels were increased in nephrotoxic condition and these levels were ing back to near normal upon treatment with quercetin and vitamin E alone or in combination through its antioxidant properties. In addition to that inflammatory markers such as TNF α, Ifi44 and IL–1 α were significantly decreased in quercetin and vitamin E alone or in combination groups.