Evaluation of Cytotoxic Potential of Various Endodontic Irrigants on Earlichs Ascitic Cells: An Invitro Study

Dr. Shreema S Shetty; Dr. Darshana Devadiga; Prof(Dr). Mithra .N hegde

Volume : IV, Issue : XII, December - 2014

Evaluation of Cytotoxic Potential of Various Endodontic Irrigants on Earlichs Ascitic Cells: An Invitro Study

Dr. Shreema S Shetty, Dr. Darshana Devadiga, Prof Dr . Mithra . N Hegde

Abstract :

The aim of this study was to investigate the in vitro cytototoxic effects of 2.5% sodium hypochlorite, 2% chlorhexidine ,2% iodine potassium iodide and physiologic saline. Earlichs ascitic cells were used invitro to evaluate the cytotoxicity of the irrigating solutions. The cells were cultured in the peritoneal cavity of Swiss Weister Mice by periodical transplantation of 0.2–0.5ml of sterile suspension in 0.9% saline(containing approx. 5x106 cells/ml). The cells were harvested 10–11 days after transplantation. Results were evaluated calculating means and standard deviations. Data were statistically analyzed by Kruskul–Wallis test (p<0.005). all the test irrigants showed variable level of cytotoxicity, with 2.5%sodium hypochlorite having the highest level of toxicity and saline having the least.