Evaluation of genotoxic potential of tannery industry  effluent in a freshwater fish, Labeo rohita via  chromosomal aberration test

Gurinder Kaur Walia; Diana Handa; Harbhajan Kaur and Rohit Kalotra

Volume : III, Issue : XII, December - 2013

Evaluation of genotoxic potential of tannery industry effluent in a freshwater fish, Labeo rohita via chromosomal aberration test

Gurinder Kaur Walia, Diana Handa, Harbhajan Kaur And Rohit Kalotra

Abstract :

Chromosomal aberration test is one of the most exclusively used biomarker to detect effects of DNA damage. In the present study, this test has been used to elucidate the genotoxic impact of tannery industry effluent on kidney tissue of Labeo rohita. 96h LC50 against fish was calculated and three sub–lethal concentrations (3.53%, 1.76% and 0.88%) were prepared. Fishes were exposed to sublethal concentrations for 24h, 48h, 72h and 96h durations. Ten types of chromosomal aberrations were observed. Out of these, Chromosome fragments, Ring chromosomes, Centromeric gaps and Minutes were predominant while Pulverization and Stretching were lowest in all the concentrations. Results indicated that higher concentration (3.53%) and exposure (96h) induced maximum chromosomal aberrations. These were statistically significant (p<0.05) as compared to control.