Evaluation of Larvicidal Activity of Tridax procumbens (Asteraceae) Leaf Extract Against the Dengue Vector, Aedes aegypti and Bancroftian Filariasis Vector, Culex quinquefasciatus

Syed Mohammed Imthiyaz Begum; Arumugam Durga; Rathinasamy Regina Mary; Berchmans Scholastica Mary Vithiya; Kuppuswamy Elumalai

Volume : IV, Issue : VIII, August - 2014

Evaluation of Larvicidal Activity of Tridax procumbens (Asteraceae) Leaf Extract Against the Dengue Vector, Aedes aegypti and Bancroftian Filariasis Vector, Culex quinquefasciatus

Syed Mohammed Imthiyaz Begum, Arumugam Durga, Rathinasamy Regina Mary, Berchmans Scholastica Mary Vithiya, Kuppuswamy Elumalai

Abstract :

The leaf extract of Tridax procumbens with different solvents Hexane, Acetone, Ethyl acetate and Methanol were tested for larvicidal activity against two important mosquitoes such as dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefasciatus. The Tridax procumbens leaves and stem powder was soaked in Hexane, Acetone, Ethyl acetate, Methanol (each solvent 1200 ml). The plant powder soaked with solvents for three days. The filtered sample was poured into a soxhlet apparatus (boiling point range 100&deg;C). After two hours, the filter paper was for concentrated at room temperature until oily paste formed and kept at cool dry place further used. From the stock solution, 500 ppm was prepared with dechlorinated tap water. The numbers of dead larvae were counted after 24 h of exposure and the percentage of mortality was recorded from the average of five replicates. The average larval mortality data were subjected to probit analysis for calculating LC50, LC90 and other statistics at 95% fiducial limits of upper confidence limit and lower confidence limit. Results with p&lt;0.05 were considered to be statistically significant. The results of the leaf extract of Tridax procumbens are promising as good larvicidal activity against the mosquito vector, A. aegypti and C. quinquefasciatus.