Volume : VIII, Issue : VIII, August - 2018

EVALUATION OF MULTIPLEX ARMS PCR FOR SCREENING OF β– THALASSEMIA MUTATIONS

Dr. Arpita Mishra, Dr. M. P Bankar

Abstract :

Introduction: β–Thalassemia is one of the most common single gene disorder worldwide, which is caused by mutations in the β globin (HBB) gene on chromosome 11. In India, the prevalence of β–Thalassemia is 2–3% in general population and as high as 17% in high risk communities. Objective:To evaluate Multiplex ARMS PCR for faster detection of β–Thalassemia mutation. Methods: ARMS PCR approach identifying 5 common β–globin gene mutations were designed and employed to investigate 30 β–thalassaemia carriers. Two β–Thalassemia mutations [IVS I–5(G–C) and Codon 8/9(+G)] were analysed using Multiplex ARMS PCR in a single reaction. Result:  With the help of Multiplex ARMS PCR   two β–Thalassemia mutations [IVS I–5(G–C) and Codon 8/9(+G)] were identified in a single reaction. Conclusion: Multiplex ARMS PCR is accurate, cost–effective, faster and labour efficient. Hence it should find a place in the routine detection of mutations of β–globin gene which can accelerate identification by mutational analysis.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dr. Arpita Mishra, Dr. M.P Bankar, EVALUATION OF MULTIPLEX ARMS PCR FOR SCREENING OF β– THALASSEMIA MUTATIONS, INDIAN JOURNAL OF APPLIED RESEARCH : Volume-8 | Issue-8 | August-2018

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