Volume : V, Issue : V, May - 2015

Evaluation of Multiplex Real–time PCR for Detection of three Diarrhea Causing Intestinal Protozoa

Mohammed Jasim Shakir

Abstract :

 Background: Diarrheal diseases are one of the leading cause‘s childhood morbidity and mortality in many  tropical and subtropical countries, in addition to have similar clinical presentation, although it remains a  diagnosis of these parasites by microscopy, but microscopy lacks sensitivity and specificity. There is many study referred  to used different immunological and molecular assay for diagnosis of parasitic infection. Objective: To evaluate a Multiplex Real–time PCR assay for the identification of Entamoeba histolytica, Giardia lamblia,  and Cryptosporidium Spp in human fecal samples.  Materials and methods: One hundred eighty stool sample collected from children attending to the central teaching  hospital for pediatric in Baghdad city (93 females and 87 males) ranging in age from 6 –12 years was collected during  the period from June 2014 to October 2014.The diagnosis was established based on direct microscopic examination of  stool and then re–diagnosed by using Multiplex Real–time PCR technique. Result: In the present study the 180 stool samples tested the PCR for the detection of Entamoeba histolytica, Giardia  lamblia, and Cryptosporidium Spp achieved a sensitivity and specificity of 100% compared to sensitivity of microscopy  ranged from 74% for Entamoeba histolytica, 84% for Giardia lamblia and 70% for Cryptosporidium Spp, Entamoeba  histolytica showed high frequency compare to Giardia lamblia and Cryptosporidium spp. Conclusion: The Multiplex Real–time PCR described here is a sensitive and specific method for the detection of intestinal protozoa. It also offers the possibility of introducing DNA detection in many laboratories and should be considered  the gold standard methods for the diagnosis of enteric protozoan disease.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Mohammed Jasim Shakir Evaluation of Multiplex Real–time PCR for Detection of three Diarrhea Causing Intestinal Protozoa Indian Journal of Applied Research, Vol.5, Issue : 5 May 2015

Number of Downloads : 530

References :