Evaluation of Radiomodulatory Effect of Haberlea  Rhodopensis (Friv.) Extract Using Micronucleus Assay  in Lymphocytes of Whole Body Irradiated Rabbits.

Svetlana Yordanova Georgieva

Volume : IV, Issue : II, February - 2014

Evaluation of Radiomodulatory Effect of Haberlea Rhodopensis (Friv.) Extract Using Micronucleus Assay in Lymphocytes of Whole Body Irradiated Rabbits.

Svetlana Yordanova Georgieva

Abstract :

Haberlea rhodopensis leaf extract (HR) possess strong antioxidant activity. The present study aimed to examine the radioprotective potentiality and efficacy of HR against DNA damage induced by γ-irradiation to dose range 1.0-3.0 Gy. HR total extract (0.12 g/kg b. w.) was injected im, into rabbits 2h before whole body irradiation. Rabbits were exposed to 1.0, 2.0 and 3.0 Gy ⁶⁰Co γ-rays. The radiation-induced changes were estimated by using cytokinesis blocked micronucleus assay (CBMN) in peripheral lymphocytes. Results reveled that the micronucleus test pointed out a significant difference between groups received HR before irradiation and γ-irradiated groups. It was concluded that HR extract was effective in the protection against clastogenic effect of radiation due to its content of different antioxidant ingredients and their ability to scavenge free radicals generated by radiation.