Evaluation Of Rapid Osom Trichomonas Test For Diagnosis Of Trichomoniasis In Females Of Reproductive Age Group

Dr. Razia Khatoon; Dr. Richa Gupta; Dr. Haris M.Khan

Volume : VII, Issue : X, October - 2017

Evaluation Of Rapid Osom Trichomonas Test For Diagnosis Of Trichomoniasis In Females Of Reproductive Age Group

Dr. Razia Khatoon, Dr. Richa Gupta, Dr. Haris M. Khan

Abstract :

Introduction:Trichonomas vaginalis is a parasitic protozoan,sexually transmitted worldwide.Diagnosis is usually done by observing motile parasite from vaginal or cervical swab specimens by direct wet mount examination,geimsa staining,fluorescent staining and culture.OSOM rapid test is newer method available for diagnosis of this sexually transmitted disease.

Methods:Study was carried out on 405 patients attending gynecological OPD with complaint of vaginal discharge.Four high vaginal swabs from posterior fornix of vagina were taken from each patient;each for wet mount,giemsa and acridine orange staining and other for OSOM trichomonas rapid test.Culture was done on kupferberg culture medium.

Results:Wet mount and giemsa staining showed T.vaginalis in 7(1.7%) cases each.Acridine orange staining was positive in 12 cases(3.0%),while OSOM rapid test detected T.vaginalis infection in 15 cases.Culture on kupferberg medium was positive in 18(4.4%) patients and was taken as gold standard.Sensitivity,specificity of wet mount examination was found to be 38.9%(CI 18.2–63.8),100%(CI 98.7–100),positive and negative predictive values,100% and 97.2%.Giemsa and acridine orange staining showed sensitivity of 38.9%(CI 18.2–63.8) and 66.67%(CI 41.4–85.6) and specificity 100 %(CI 98.7–100) each.OSOM rapid test was found to be most sensitive(83.3%,CI 57.7–95.5 ) when compared to wet mount examination and staining techniques taking while staining methods and OSOM test were found to be equally specific(100%,CI 98.7–100).

Conclusion:OSOM rapid test is simple to perform,objective and can provide results in 10 min.It will be an important addition to the repertoire of techniques available for T.vaginalis detection,especially for facilities without access to a microscope or incubator and in settings where difficult patient follow–up makes point–of–care testing attractive.