IJAR - Indian Journal of Applied Research

Nishtha K. Singh Dr. Umesh Luthra Dr. Neetin Desai

Volume : III, Issue : VII, July - 2013

Evaluation of Vitamin B12 Synthesis by isolated Rhizobium sp. from Sesbania sesban found in Mumbai and its suburban areas

Nishtha K. Singh Dr. Umesh Luthra Dr. Neetin Desai

Abstract :

ABSTRACT Vitamin B12 is most chemically complex of all the Vitamins .It was produced in 1926, as an antipernicious anemia factor. Its synthesis is restricted to micro–organism which engrossed complicated biosynthetic pathway. In present study Vitamin B12 production was studied with newly isolated Rhizobium species obtained from Sesbania sesban. The six isolates of Rhizobium spp were screened for the production of Vitamin B12. Submerged fermentation was applied for the evaluation of Vitamin B12 Synthesis. All six isolates were capable of producing Vitamin B12, on an average range of approx 2–3 mg/kg. Isolate CHA was able to produce higher yield of 1.35 mg/kg in comparison to control culture (Pseudomonas denitrificans ATCC 19244) 1.05 mg/ kg. Maximum activity of 5.08 mg/kg was attained with CHA culture by media optimization with literature media while in control culture the activity was mg/kg. Production of Vitamin B12 depends on the proper balance of medium constituents and their utilization by isolates during the fermentation process. Addition of Beet molasses in fermentation medium shows positive impact on Vitamin B12 Yield.