EVALUTION OF LEUCONOSTOC MESENTERIODES., AGAINST BACTERIAL WHITE SPOT DISEASE INDUCED BY VIBRIO CHOLERAE IN SHRIMPS

A. Sathya Priya; A. Anitha; S. Parasmal

Volume : VIII, Issue : IX, September - 2018

EVALUTION OF LEUCONOSTOC MESENTERIODES., AGAINST BACTERIAL WHITE SPOT DISEASE INDUCED BY VIBRIO CHOLERAE IN SHRIMPS

A. Sathya Priya, A. Anitha, S. Parasmal

Abstract :

The shrimp disease in culture pond mainly accounted as a Viiosis caused by Viio sp. Probiotic supplementation of live microorganism in aquaculture aids in preventing disease, thereby increasing production and decreasing economic loss. The use of probiotics or beneficial bacteria, which control pathogen through a variety of mechanisms, is increasingly viewed as an alternative to antibiotic treatment. The present study was aimed to determine the probiotic activity of Leuconostoc mensenteroides (MCC NO 3276) against Viiocholerae., in aquaculture. Traditional bacterial identification has been based on different phenotypic characteristics. Invitro antagonist activities were tested by dual culture, cross streak method, SEM analysis. The study concludes that invitro antagonistic assay of dual culture test with L. mensenteroides clearly exhibited the growth inhibition of V. cholerae. In co–culture experiment, growth of pathogenic V.cholerae was inhibited by L. mensenteroides culture inoculated at equal ratio even in low ratio was also controlled the V. cholerae under invitro condition. Morphological analysis by SEM revealed that V.cholerae treated with L. mensenteroides showed major structural disruption in the cell envelope as well as a preponderance as irregular rods forms than the controls.