Explants Size Response to in Vitro Propagation of  Musa (Aaa Group) �Amritsagar� Musa (Aab Group)  �Malbhog� and Musa (Aab Group) �Chenichampa�  Banana

N K Goswami; P. J. Handique

Volume : III, Issue : VIII, August - 2013

Explants Size Response to in Vitro Propagation of Musa (Aaa Group) �Amritsagar� Musa (Aab Group) �Malbhog� and Musa (Aab Group) �Chenichampa� Banana

N K Goswami, P. J. Handique

Abstract :

In vitro propagation of Musa (AAA group) ‘Amritsagar’, Musa (AAB group) ‘Malbhog’ and Musa (AAB group) ‘Chenichampa’ was carried out using sword sucker explants. The effect of three different sizes of explants (5, 10 and 20 mm) on the establishment of banana in micropropagation was investigated. Three cultivars (Amritsagar, Malbhog and Chenichampa) were used for the study with an average of 12 explants per treatment per cultivar. Initiation media– MS Basal, MS+BAP 0.2 mg L–1, MS+BAP 0.3 mg L–1and MS+BAP 0.5 mg L–1 were used to evaluate the explants size response for initiating and establishing banana cultures in in vitro condition. The larger explants (20 mm) responded well with regard to survival of explants, days to swelling and greening of explants, emergence of leaf and days to multiple bud initiation under in vitro condition as compared to smaller explants.