Exploitation of Parthenium Hysterophorus L. for  the Rapid Biosynthesis of Silver Nanoparticles and  Evaluation of their Anti–Microbial Activity

Dr.Ananda S

Volume : III, Issue : VII, July - 2013

Exploitation of Parthenium Hysterophorus L. for the Rapid Biosynthesis of Silver Nanoparticles and Evaluation of their Anti–Microbial Activity

Dr. Ananda S

Abstract :

The improvement of rapid and reliable processes for the synthesis of nanosized materials is of great importance in the field of nanotechnology. In this paper, we describe a novel synthesis approach which is rapid, simple and “green” for the synthesis of metallic nanostructures of noble metals such as silver, by using plant extract of Parthenium hysterophorus L treated with 1mM AgNO3 solution and microwave irradiation. It was found that exposure of plant extract of Parthenium hysterophorus L and microwave irradiation to silver ion lead to the formation of silver nanoparticles. The nanoparticles were examined using UV–Visible Spectroscopy, X Ray diffraction, Scanning electron microscopy, and Transmission Electron Microscopy analyses. The formation of nanoparticles by this method is extremely rapid, requires no toxic chemicals and the nanoparticles are stable for few months. Further these biologically synthesized nanoparticles were found to be toxic against different human pathogens namely Escherichia coli L, Pseudomonas aeruginosa L, Klebsiella pneumoniae L. The antimicrobial activity of the silver nanoparticles was determined. Scanning Electron Microscopy micrograph analysis and Transmission Electron Microscopy of the silver nanoparticles indicated that they were well–dispersed and the size was found to be around 10nm.