Extraction and physico–chemical analysis of chitosan from shell of marine crab Scylla serrata.

Dinesh Wanule; Kantilal H. Nagare; Sariya Moulvi; Nitin P. Koche

Volume : VI, Issue : III, March - 2016

Extraction and physico–chemical analysis of chitosan from shell of marine crab Scylla serrata.

Dinesh Wanule, Kantilal H. Nagare, Sariya Moulvi, Nitin P. Koche

Abstract :

 Chitosan is deacetylated form of chitin. It is obtained from various sources like fishes, molluscans, arthropods and fungi. Chitosan has diverse industrial applications. The present study was carried out for extracting Chitosan from carapace of locally available crab Scylla serrata. The study was conducted in five replicates. The mean of obtained Chitosan was 1.28gm Chitosan per 3 gram of carapace. Extracted Chitosan was subjected to FTIR, SEM and EDAX analysis. The spectra has characteristic band for Chitosan. The FTIR spectral analyses showing a peak at 3396.99 cm–1 indicate symmetric stretching viation for OH group and amide group. Absorption peak at 2932.23cm–11 indicate presence of CH stretch , Absorption band at 1594.84cm–1 amide I and 1398.14 was due to C–H stretching and 872.631 cm–1– is a ring stretching a characteristic bond for β–1–4 glycosidic linkage. SEM Studies showed that Chitosan has smooth, nonporous, plane texture with particle size ranging from 3.05 µm to 10.82 µm.