Extraction, Isolation and Chemical Structure  Elucidation of Isorhamnetin From Leaves and Stems  of Argemone Mexicana Linn of Bhopal, Madhya  Pradesh, India

Saurabh  Rajvaidhya; Gajendra  Kamal  Singh; Badri  Prakash  Nagori; Saroj  Kumar  Pradhan

Volume : IV, Issue : II, February - 2014

Extraction, Isolation and Chemical Structure Elucidation of Isorhamnetin From Leaves and Stems of Argemone Mexicana Linn of Bhopal, Madhya Pradesh, India

Saurabh Rajvaidhya, Gajendra Kamal Singh, Badri Prakash Nagori, Saroj Kumar Pradhan

Abstract :

PURPOSE: To extract, isolate and identify isorhamnetin from the stems and leaves of Argemone mexicana Linn of Bhopal, Madhya Pradesh, India. METHODS: Ethyl acetate soluble fraction of successive methanol extract from the stems and leaves of A. mexicana was subjected to column chromatography and fractionated by benzene, n-butanol, and acetone successively. Eluents having similar Rf values were pooled together. These pools were subjected to flavonoid test and only a single pool passed it which was again subjected to chromatographic separation for purification and crystallized powers were subjected to FT-IR, EI-MS and 1H-NMR for structure identification. CONCLUSION: From this study it can be concluded that the whole plant (except roots and capsules) can be used as a source of isorhamnetin.