Genetic diversity analysis of crab spider (Araneae: thomisidae) based on RAPD–PCR

Nandkishor Warghat; Navin Sharma; Punam Thakur; Mumtaz Baig

Volume : IV, Issue : VIII, August - 2014

Genetic diversity analysis of crab spider (Araneae: thomisidae) based on RAPD–PCR

Nandkishor Warghat, Navin Sharma, Punam Thakur, Mumtaz Baig

Abstract :

The current study deals with the genetic diversity of crab spiders using molecular markers. Total scorable bands were produced using six random primers for the 16 species of crab spiders belonging to family thomisidae. Out of all screened primers, OPP 9 produced highest 113 scorable bands, out of which 111 bands were found with 98.28 percent polymorphism. Primer OPA 3 was produced lowest 43 bands with 100 percent polymorphism. Remaining primers (OPA 2, OPA 4, OPN 16 and OPN 18) showed 100 per cent polymorphism with 60, 60, 81 and 51 bands respectively. Along with the molecular markers, a morphometric analysis was also focus on the phylogenetic relationship of crab spiders using UPGMA and NJ approach. The present study is the first report from India to describe the genetic relatedness amongst spider using RAPD–PCR. 