Genetic Variation in Aedes Aegypti Mosquito Populations Along the West Cost of India and Their Susceptibility to Insecticides and Dengue Virus

Mourya DT; Kumar R; Barde PV; Gokhale MD; Yadav PD

Volume : V, Issue : I, January - 2015

Genetic Variation in Aedes Aegypti Mosquito Populations Along the West Cost of India and Their Susceptibility to Insecticides and Dengue Virus

Mourya Dt, Kumar R, Barde Pv, Gokhale Md, Yadav Pd

Abstract :

Genetic characteristics of twelve Aedes aegypti mosquito populations collected from differnt geographical areas along the westcoast of India was studied using bioassays, biochemical assays and dengue–2 virus susceptibility. Polymorphism in the populations was also studied using random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR), amplification of mitochondrial DNA fragments from cytochrome–b gene and isoenzyme profile. Resistance to DDT and Malathion was detected in majority of strains while none developed deltamethrin resistance. Dengue–2 virus infection rates among the populations ranged between 13.8% and 47.2%. Isoenzyme profile and RAPD–PCR studies demonstrated a higher genetic heterozygosity among the populations at 19 presumptive loci. Phylogenetic analysis based on cytochrome–b gene identified three well–supported clades. The study neither showed any association of genetic composition with insecticide resistance or dengue–2 virus susceptibility nor any association could be demonstrated for insecticide susceptibility staus and dengue virus susceptibility