Volume : IX, Issue : II, February - 2019

Genotyping of Hepatitis B virus (HBV) by RFLP among clinical groups in chronic hepatitis B patients in North India

Mohammad Suhaib, Meher Rizvi, M. Azam, M. R. Ajmal

Abstract :

AIM: To study the distribution of Hepatitis B virus (HBV) genotypes among various clinical groups in chronic hepatitis B patients in a North Indian tertiary care centre. MATERIALS AND METHODS: Consecutive samples were collected from 50 HBsAg positive cases which were divided into four clinical groups namely, a)Chronic viral hepatitis B(CVH), b)Acute exacerbation of chronic hepatitis B virus (CHB–AE), c)Incidentally Detected Asymptomatic HBsAg Positive Subjects(IDAHS) and d) Hepatocellular Carcinoma(HCC). Amplification of surface gene was performed by nested PCR using specific primers and results were confirmed on gel electrophoresis. Genotyping was done on amplified product by RFLP using NlaIV and specific bands were analysed. RESULTS: Only genotypes A and D were observed and genotype D was predominated. Out of 50 patients genotype D was present in 49(98%) of chronic HBV patients. Among genotype D patients 36(73.5%) suffered from CVH, 4 (8%) from CHB–AE, 8(16.3%) were Incidentally Detected Asymptomatic HBsAg Positive Subjects (IDAHS) and 1(2%) from Hepatocellular Carcinoma. Genotype A was present in 1(2%) patient which was from CVH disease group. CONCLUSION: Only HBV genotypes A and D were found in patients with chronic hepatitis B. No coinfections with genotype A and D were seen. Genotype D was the dominant genotype observed among the groups. During our study RFLP proved to be helpful tool owing to its high sensitivity and specificity. NlaIV can be used as a preliminary restriction endonucleases as it efficiently differentiates between genotype A and D on the basis band patterns, which are predominant genotypes in the region. Other restriction enzymes like Alwl, Earl, Hphl, Ncil may be used in consecutive step for determination of other genotypes.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

GENOTYPING OF HEPATITIS B VIRUS (HBV) BY RFLP AMONG CLINICAL GROUPS IN CHRONIC HEPATITIS B PATIENTS IN NORTH INDIA, Mohammad Suhaib, Meher Rizvi, M.Azam, M. R. Ajmal INDIAN JOURNAL OF APPLIED RESEARCH : Volume-9 | Issue-2 | February-2019

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