GRAM‘S STAINING OF TISSUE BACTERIA: A CASE REPORT AND A COMPARISON OF THREE MODIFICATIONS OF THE TECHNIQUE

Dr.Heera.R; Dr Padmakumar S K; Dr.Anu Andrews; Dr.Divya K.T; Dr.Shinu Koshy

Volume : VIII, Issue : VIII, August - 2018

GRAM‘S STAINING OF TISSUE BACTERIA: A CASE REPORT AND A COMPARISON OF THREE MODIFICATIONS OF THE TECHNIQUE

Dr. Heera. R, Dr Padmakumar S K, Dr. Anu Andrews, Dr. Divya K. T, Dr. Shinu Koshy

Abstract :

There are many microorganisms in our environment, but everybody is not susceptible to such organism. Only people who are immunocompromised are usually vulnerable to the Infections caused by these microorganism. In most of the cases these organisms are missed in routine H and E section due to lack of experience of the pathologist, hence here comes the importance of special stains. In our study we compared 3 modifications of Grams staining of tissue–Brown– Hopp’s Gram Stain Method, Churukian’s modification and Gram Twort stain and concluded that Brown–Hopp’s method showed superior staining results. Even though newer technique are now flourished older technique are also used due to lack of infrastructure and cost factor. We did a comparison between 3 stains and our aim was to find out a superior stain among them.