HEADSPACE GAS CHROMATOGRAPHY- MASS SPECTROMETRY (GC-MS) AND IT'S APPLICATIONS-A REVIEW

Dr. K. Bhavyasri; G.Srihitha; Dr. D. Rambabu

Volume : IX, Issue : III, March - 2019

HEADSPACE GAS CHROMATOGRAPHY- MASS SPECTROMETRY (GC-MS) AND IT'S APPLICATIONS-A REVIEW

Dr. K. Bhavyasri, G. Srihitha, Dr. D. Rambabu

Abstract :

Headspace gas chromatography–mass spectrometry (GC–MS) is a technique used for the concentration and analysis of volatile organic compounds. This technique is relatively simple, fastest and cleanest method that can provide sensitivity similar to dynamic purge and trap analysis. A headspace sample is normally prepared in a vial containing the sample, the dilution solvent, a matrix modifier. Water or a high–boiling organic solvent may be added to dissolve the sample and facilitate release of volatile compounds. The vials are typically heated to drive partitioning of volatiles from the solid or liquid into gas phase which are to be agitated during heating. An aliquot of the vapour in the headspace is delivered to a GC system for separation of all the volatile components. The popularity of this technique has grown and has gained worldwide acceptance for analysis of alcohols in blood and residual solvents in pharmaceutical products